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KMID : 0613820100200111617
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Journal of Life Science
2010 Volume.20 No. 11 p.1617 ~ p.1624
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Jang Ji-Yeon
Kim Ha-Neui
Kim Yu-Ri
Kim Byung-Woo
Choi Yung-Hyun
Choi Byung-Tae
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Abstract
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Recently, it has been found that Asiasari radix showed a hypopigmenting effect on melanogenesis through activation of mitogen-activated protein kinase (MEK)/extracellular signal-activated kinase (ERK) in B16F10 melanoma cells. However, the hypopigmenting effect of A. radix on the α-melanocyte stimulating hormone (α-MSH)-stimulated melanogenesis has remained unknown. The purpose of this study was to investigate the inhibitory mechanism of the partially purified A. radix (PPAR)-induced hypopigmentating effects on α-MSH-stimulated melanogenesis in B16F10 mouse melanoma cells. PPAR strongly inhibited tyrosinase activity and leads to decreased melanin synthesis in α-MSH-stimulated B16F10 melanoma cells. PPAR also decreased the α-MSH-induced over-expression of the melanogenic enzymes, tyrosinase, tyrosinase-related protein (TRP)-1, dopachrome tautomerase (Dct) and microphthalmia-associated transcription factor (MITF). We further showed that PPAR inhibits α-MSH-induced melanogenesis via phosphorylation of MEK/ERK and PI3K/Akt, and that their activation was blocked by MEK inhibitors, PD98059 and PI3K inhibitors, LY294002 in α-MSH-stimulated B16F10 melanoma cells. These results suggest that PPAR inhibits α-MSH-induced melanogenesis by activation of MEK/ERK and PI3K/Akt through MITF degradation, which may lead to down-regulation of tyrosinase.
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KEYWORD
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Asiasari Radix, α-melanocyte stimulating hormone (α-MSH), microphthalmia-associated transcription factor (MITF), mitogen-activated protein kinase (MEK)/extracellular signal-activated kinase (ERK), PI3K/Akt
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